| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium perfringens | TGCCTGCACTTATGGGAGC | GCTGCTGGCCATACTAAAGC | 60.08 | 59.33 | 160 | 166 |
100.00%
|
25.00%
|
| Primer 2 | Clostridium perfringens | GGGCTAGAGCATATTACGTGC | TCCACCATGAGCCTCTTGG | 58.66 | 59.01 | 248 | 158 |
100.00%
|
25.00%
|
| Primer 3 | Clostridium perfringens | TTCAGTTGAATGGTGTGGACC | AACGGCTACTGGCACTGATC | 58.42 | 60.11 | 151 | 141 |
100.00%
|
25.00%
|
| Primer 4 | Clostridium perfringens | TTCAGTTGAATGGTGTGGACC | TAACGGCTACTGGCACTGATC | 58.42 | 59.86 | 152 | 141 |
100.00%
|
25.00%
|
| Primer 5 | Clostridium perfringens | GGGCTAGAGCATATTACGTGC | GTCCACCATGAGCCTCTTGG | 58.66 | 60.39 | 249 | 138 |
100.00%
|
25.00%
|
| Primer 6 | Clostridium perfringens | GCGTGGGATGCTTAACATGC | TGGGCAAGCCATTACACAC | 60.25 | 58.35 | 242 | 138 |
100.00%
|
25.00%
|
| Primer 7 | Clostridium perfringens | GCGTGGGATGCTTAACATGC | ATGGGCAAGCCATTACACAC | 60.25 | 58.81 | 243 | 138 |
100.00%
|
25.00%
|
| Primer 8 | Clostridium perfringens | TCCAGGATTTAGGGGTGCTG | AGGCTTCAGCTGTGCTTCC | 59.08 | 60.30 | 239 | 132 |
100.00%
|
25.00%
|
| Primer 9 | Clostridium perfringens | TTCAGTTGAATGGTGTGGACC | AACGGCTACTGGCACTGAT | 58.42 | 59.02 | 151 | 131 |
100.00%
|
25.00%
|
| Primer 10 | Clostridium perfringens | GTGCTAGAAGCCGATGCAG | GCTACCAACAACGTCGAATCC | 58.69 | 59.61 | 216 | 131 |
100.00%
|
25.00%
|